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All Journal HAYATI Journal of Biosciences Agroland : Jurnal Ilmu-Ilmu Pertanian Jurnal Matematika & Sains Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Rizkita Rachmi Esyanti
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Mathematics

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Journal : Jurnal Matematika

 1 
Pengaruh Pemberian Elisitor Homogenat Jamur Pythium aphanidermatum (Edson) Fitzp. terhadap Kandungan Ajmalisin dalam Kultur Akar Catharantus roseus (L) G. Don Mukarlina Mukarlina; Rizkita Rachmi Esyanti; Arbayah Hamonangan Siregar
Jurnal Matematika & Sains Vol 11, No 2 (2006)
Publisher : Institut Teknologi Bandung

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An experiment to study the effect of elicitor from Pythium aphanidermatum (Edson) Fitzp. on ajmalicine content of Catharanthus roseus (L) G. Don. root culture has been conducted. The C. roseus root culture in Zenk medium (1977) with the the addition of 10-7 M of napthalene acetic acid (NAA) and 10-7 M of benzylaminopurine (BAP) as growth factors was elicited with autoclaved P. aphanidermatum homogenate at concentrations of 0.05, 0.5, 1.0 and 50 mg DW/mL, then harvested at 18, 24, 36, 48, and 72 h after elicitation. The ajmalicine content was analyzed qualitatively and quantitatively by HPLC (High Pressure Liquid Chromatography) connected to chromatopack CR-7A plus. The result showed that ajmalicine content was influenced significantly by the concentration of elicitor and harvesting time. The highest ajmalicine content on the roots i.e. 165.6 ± 1.1 μg/g DW with the increment percentage of 181,6% was obtained by addition of 5.0 mg DW/mL of elicitor for 24 h, while on the media, the highest ajmalicine content i.e. 291.5 ± 0.9 mg DW/mL with the increment percentage of 306,5% was obtained by addition of 5.0 mg DW/mL elicitor for 36 hours. These results indicated that elicitation might be responsible for the higher ajmalicine content in the media compared to its accumulation in the root.
Pengaruh Triptofan pada Pertumbuhan dan Kandungan Katarantin dari Kalus Catharanthus roseus Dingse Pandiangan; Dennie Heroike Rompas; Henry Fonda Aritonang; Rizkita Rachmi Esyanti; Erly Marwan
Jurnal Matematika & Sains Vol 11, No 4 (2006)
Publisher : Institut Teknologi Bandung

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Research on the growth and catharanthine content of Catharanthus roseus callus which were treated with tryptophan had been done. Wet and dry weight of Catharanthus roseus callus decreased when treated with 100, 125 and 225 mg/L tryptophan, but callus treated with 150, 175 and 200 mg/L tryptophan grew better. Catharanthine contents of the kontrol callus was 423,22 µg/g dw. The treatment calli contains 485,00, 588,32, 875,10, 905,26, 784,10 and 950,54 µg/g dw of catharanthine respectively. The optimal tryptophan treatment for callus growth and catharanthine production was 175 mg/L tryptophan with a 113,90 %. increase of catharanthine content.
Effect of 2,4-D on Indirect Somatic Embryogenesis and Surface Structural Changes in Garlic (Allium sativum L.) cv. Lumbu Hijau Totik Sri Mariani; Hiroshi Miyake; Rizkita Rachmi Esyanti; Ida Nurwendah
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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A study concerning the effect of 2,4-D on indirect somatic embryogenesis and surface structural changes in garlic (Allium sativum L.) cv. Lumbu Hijau was conducted. The purposes of this study were to evaluate the effect of 2,4-D on the induction of somatic embryogenesis and to observe the developmental stage of somatic embryo as well as the surface structural changes of somatic embryo in garlic. Root tips explants were cultured on embryogenic callus induction medium (ECIM). Somatic embryos were then transferred to the embryo maturation medium (EMM), desiccated and subsequently transferred to the repetitive somatic embryogenesis medium (RSEM). Observation by dissecting microscope showed that embryogenic callus and somatic embryo was formed at ECIM containing 0.1 μM 2,4-D. At EMM containing 0.01 μM 2,4-D somatic embryo developed into mature somatic embryo. Somatic embryo underwent repetitive somatic embryogenesis that consisted of globular and mature somatic embryo at RSEM, without plant growth regulator. Observation by scanning electron microscopy showed that the development al stages of garlic somatic embryo consisted of proembryo, transition phase from proembryo to globular, globular, mature embryo and single cotyledon stages. Effect of 2,4-D on the cell surface structure could be seen on each somatic embryo developmental stage. In ECIM containing 0,1 μM 2,4-D, proembryo, transition embryo and globular embryo were formed. The surface of proembryo was smooth whereas fibrillar material as cellulose microfibril, was observed during the transition from proembryo to globular stage. The surface of globular stage was entirely covered by a new cell wall. Subsequently, in 0.01 μM 2,4-D, mature embryo and single cotyledon were formed, which their cell wall surface was stable. From this study, it could be concluded that the optimum concentration of 2,4-D for induction of embryogenic callus and initiation of somatic embryo was 0.1 μM. Decreasing of 2,4-D concentration to 0.01 μM resulted in the development of globular embryo to mature and single cotyledon stage. Repetitive somatic embryogenesis occurred in RSEM medium without plant growth regulator. Based on this result it can be concluded that each somatic embryo developmental stage had a typical characteristic of surface structure.
Co-Authors AIDA MUSPIAH Anak Agung Istri Sri Wiadnyani Arbayah H Siregar Arbayah Hamonangan Siregar Dennie Heroike Rompas Dingse Pandiangan Erly Marwan Henry Aritonang Hiroshi Miyake I Nyoman P. Aryantha Ida Nurwendah Suci Rahayu Tjandra Anggraini Totik Sri Mariani Umrah Umrah